A modified acid-fast staining method was developed for rapid detection of Mycobacterium tuberculosis and its L forms, wherein carbol fuchsin and dioxogen were mixed into the sputum smear. With this method, the dyeing time is shortened and heating is not required.
Acid-Fast Staining Instructions
AFB stain (Ziehl-Neelsen acid-fast) staining procedure:
Fluorochrome acid-fast microscopy is easy to perform and it's currently the most rapid procedure for detecting AFB in clinical specimens . Fluorochrome-stained smears can be viewed at lower magnifications.
There are two main types of AFB tests:
If the site must be touched during venipuncture, disinfect the gloved fingers. 5. Collect 5-10 mL of blood and aseptically inoculate the green top tube .
The AFB culture refers to the process of inoculating a clinical specimen onto culture media ; including Becton-Dickinson Mycobacteria Growth Indicator Tubes (B-D MGIT broth) and a Lowenstein-Jensen (L-J) media slant. It is then incubated at 37°C for up to six weeks. Lastly, it is examined for growth or no growth.Mar 16, 2021
Take a very deep breath and hold the air for 5 seconds. Slowly breathe out. Take another deep breath and cough hard until some sputum comes up into your mouth. Spit the sputum into the plastic cup.
There are two kinds of tests that are used to detect TB bacteria in the body: the TB skin test (TST) and TB blood tests . A positive TB skin test or TB blood test only tells that a person has been infected with TB bacteria.